PUMA antibody (AA 131-180)

Catalog No. ABIN731528

The Rabbit Polyclonal anti-PUMA antibody has been validated for WB, ELISA, IP, FACS, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect PUMA in samples from Human, Rat and Pig. There are 3+ publications available.

Quick Overview for PUMA antibody (AA 131-180) (ABIN731528)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reactivity: Human, Rat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUMA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PUMA Antibody

Binding Specificity: AA 131-180

Cross-Reactivity: Human, Pig, Rat

Predicted Reactivity: Mouse,Dog,Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PUMA

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
IP(1-2 μg)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-PUMA antibody (ABIN731528)

Su, Shi, Yan, Xi, Su: "Anticancer bioactive peptides suppress human colorectal tumor cell growth and induce apoptosis via modulating the PARP-p53-Mcl-1 signaling pathway." in: Acta pharmacologica Sinica, Vol. 36, Issue 12, pp. 1514-9, (2016) (PubMed).

Qiu, Cai, Gao, Gu, Liu: "A small peptide derived from p53 linker region can resume the apoptotic activity of p53 by sequestering iASPP with p53." in: Cancer letters, Vol. 356, Issue 2 Pt B, pp. 910-7, (2014) (PubMed).

Zhang, Liu, Xia, Zhang, Zhang, Zhao, Chao, Jiang, Jiang: "Epithelial-mesenchymal transition is necessary for acquired resistance to cisplatin and increases the metastatic potential of nasopharyngeal carcinoma cells." in: International journal of molecular medicine, Vol. 33, Issue 1, pp. 151-9, (2013) (PubMed).

Target Details for PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Alternative Name: PUMA

Background:

Synonyms: JFY1, PUMA, JFY-1, Bcl-2-binding component 3, p53 up-regulated modulator of apoptosis, BBC3

Background: Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Isoform 3 fails to show any growth-inhibitory or apoptotic activity.

Gene ID: 27113

UniProt: Q96PG8

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity