PUMA antibody (AA 131-180) (Cy7)

Catalog No. ABIN731534

The Rabbit Polyclonal anti-PUMA antibody has been validated for WB, FACS, IF (cc) and IF (p). It is suitable to detect PUMA in samples from Human, Rat and Pig.

Quick Overview for PUMA antibody (AA 131-180) (Cy7) (ABIN731534)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reactivity: Human, Rat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUMA antibody is conjugated to Cy7

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-PUMA Antibody (Cy7)

Binding Specificity: AA 131-180

Cross-Reactivity: Human, Pig, Rat

Predicted Reactivity: Mouse,Dog,Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PUMA

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Alternative Name: Puma

Background:

Synonyms: JFY1, PUMA, JFY-1, Bcl-2-binding component 3, p53 up-regulated modulator of apoptosis, BBC3

Background: Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Isoform 3 fails to show any growth-inhibitory or apoptotic activity.

Gene ID: 27113

UniProt: Q96PG8

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity