CD46 antibody (CF®740)

Catalog No. ABIN7651622

The Mouse Monoclonal anti-CD46 antibody has been validated for FACS. It is suitable to detect CD46 in samples from Human.

Quick Overview for CD46 antibody (CF®740) (ABIN7651622)

Target: CD46

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD46 antibody is conjugated to CF®740

Application: Flow Cytometry (FACS)

Clone: 122-2

Product Details anti-CD46 Antibody (CF®740)

Purpose: CD46(122.2), CF740 conjugate

Characteristics: CD46 acts as a cofactor for complement factor I, a serine protease, which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. CD46 acts as a co-stimulatory factor for T-cells, which induces the differentiation of CD4 into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human CD46 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HeLa, K-562 or MOLT-4 cells. Kidney

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD46

Target: CD46

Alternative Name: CD46

Background:

Synonyms: AHUS2, Complement membrane cofactor protein, MCP, Measles virus receptor, Membrane cofactor protein, MIC10, TLX, Trophoblast leucocyte common antigen, Trophoblast lymphocyte cross reactive antigen

Gene Symbol: CD46

Molecular Weight: 56-66 kDa

Gene ID: 4179

UniProt: P15529

Pathways: Regulation of Actin Filament Polymerization