DPP4 antibody (CF®740)

Catalog No. ABIN7653186

This anti-DPP4 antibody is a Mouse Monoclonal antibody detecting DPP4 in FACS, IF and Func. Suitable for Human and Rat.

Quick Overview for DPP4 antibody (CF®740) (ABIN7653186)

Target: DPP4 (Dipeptidyl-Peptidase 4 (DPP4))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This DPP4 antibody is conjugated to CF®740

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Clone: 202-36

Product Details anti-DPP4 Antibody (CF®740)

Purpose: CD26(202-36), CF740 conjugate

Characteristics: Recognizes a glycoprotein of 110 kDa, identified as CD26 (Workshop VI, Code: N-L039). It is an atypical serine protease belonging to the prolyl oligopeptidase family. It is expressed on lymphocyte cells and is upregulated during T-cell activation. CD26 is also expressed on activated B cells and natural killer cells and abundantly on epithelia. CD26 is implicated in a variety of biological functions including T-cell activation, cell adhesion with extracellular matrix such as fibronectin or collagens, and in HIV infection. Cross-linking of CD26 using this antibody dramatically enhances the anti-CD3-induced IL-2 production. In Western blotting, this MAb reacts with only glycosylated CD26, but not with the deglycosylated form. It does not prevent ADA binding to CD26.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human T cell clone

Isotype: IgG2b, kappa

Application Details

Application Notes: Immunohistology Frozen Only 0.5-1.0 μg/mL. Immunofluorescence 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Does not react with pig or sheep, others not tested. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: YT, HEP-G2 cells, and lymphocytes. Lymph nodes and tonsils.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for DPP4

Target: DPP4 (Dipeptidyl-Peptidase 4 (DPP4))

Alternative Name: CD26

Background:

Synonyms: ADA-binding protein (ADABP), Adenosine deaminase complexing protein 2 (ADCP-2), Dipeptidyl peptidase 4, Dipeptidyl peptidase 4 soluble form, Dipeptidyl peptidase IV membrane form, Dipeptidyl peptidase IV soluble form, Dipeptidyl peptidase, intestinal, Dipeptidylpeptidase 4, Dipeptidylpeptidase IV, DPP4, DPPIV, Intestinal dipeptidyl peptidase, T-cell activation antigen CD26, TP103

Gene Symbol: DPP4

Tissue Expression: Epithelial cells

Molecular Weight: ~110 kDa

Gene ID: 1803

UniProt: P27487

Pathways: Peptide Hormone Metabolism, Regulation of Leukocyte Mediated Immunity