Recombinant CD15 antibody (CF®740)

Catalog No. ABIN7654226

The Rabbit Monoclonal anti-CD15 antibody has been validated for FACS, IHC, IF and IHC (fp). It is suitable to detect CD15 in samples from Human, Mouse and Rat.

Quick Overview for Recombinant CD15 antibody (CF®740) (ABIN7654226)

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD15 antibody is conjugated to CF®740

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: FUT4-1478R

Product Details anti-CD15 Antibody (CF®740)

Purpose: CD15 / FUT4 (Reed-Sternberg Cell Marker) (FUT4/1478R), CF740 conjugate

Characteristics: CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin's disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified human neutrophils

Isotype: IgG, kappa

Application Details

Application Notes: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min. Flow cytometry 2-4 μg/million cells. Immunofluorescence 2-4 μg/mL. Immunohistology (formalin) 5-10 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: U937 cells, Reed-Sternberg cells in Hodgkin lymphoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD15

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Alternative Name: CD15

Background:

Synonyms: CD162, CLA, Cutaneous lymphocyte associated antigen, P-selectin glycoprotein ligand 1, PSGL1, Selectin P ligand, SELPLG

Gene Symbol: FUT4

Tissue Expression: Granulocytes

Molecular Weight: 220 kDa

Gene ID: 2526

UniProt: P22083