Recombinant Insulin antibody (CF®647)

Catalog No. ABIN7655291

The Rabbit Monoclonal anti-Insulin antibody has been validated for IHC and IHC (fp). It is suitable to detect Insulin in samples from Human, Pig, Mouse, Cow and Rabbit.

Quick Overview for Recombinant Insulin antibody (CF®647) (ABIN7655291)

Target: Insulin (INS)

Antibody Type: Recombinant Antibody

Reactivity: Human, Pig, Mouse, Cow, Rabbit

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Insulin antibody is conjugated to CF®647

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: IRDN-1980R

Product Details anti-Insulin Antibody (CF®647)

Purpose: Insulin / IRDN (beta-Cell & Insulinoma Marker) (IRDN/1980R), CF647 conjugate

Characteristics: This antibody recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length Insulin protein (IRDN)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: MIA PaCa-2 cells. Pancreas.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: Insulin

Background:

Synonyms: IDDM2, ILPR, IRDN, MODY10, Proinsulin

Gene Symbol: INS

Tissue Expression: Pancreatic beta cells

Molecular Weight: 6 kDa

Gene ID: 3630

UniProt: P01308

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation