Recombinant IGF1 antibody

Catalog No. ABIN7655348

The Rabbit Monoclonal anti-IGF1 antibody has been validated for FACS. It is suitable to detect IGF1 in samples from Human, Mouse, Rat and Rabbit.

Quick Overview for Recombinant IGF1 antibody (ABIN7655348)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat, Rabbit

Host: Rabbit

Clonality: Monoclonal

Conjugate: This IGF1 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: IGF1-2872R

Product Details anti-IGF1 Antibody

Purpose: Insulin-like Growth Factor-1 (IGF-1) (IGF1/2872R)

Characteristics: This antibody is specific to Insulin-like Growth Factor (IGF-1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin. IGF-1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human IGF-1 protein

Isotype: IgG

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. ELISA For coating, order Ab without BSA. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Pancreas or brain. Breast, Thyroid or Colon Cancers, IGF-1 recombinant protein

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Alternative Name: Insulin-like Growth Factor-1

Background:

Synonyms: Insulin-like Growth Factor IA (IGF-IA), Insulin-like Growth Factor IB (IGF-IB), Mechano Growth Factor (MGF), Somatomedin-C

Gene Symbol: IGF1

Molecular Weight: ~7.6 kDa

Gene ID: 3479

UniProt: P05019

Pathways: RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation