PLK1 antibody (CF®594)

Catalog No. ABIN7658763

This anti-PLK1 antibody is a Mouse Monoclonal antibody detecting PLK1 in WB and IP. Suitable for Xenopus laevis.

Quick Overview for PLK1 antibody (CF®594) (ABIN7658763)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivity: Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This PLK1 antibody is conjugated to CF®594

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: AZ44

Product Details anti-PLK1 Antibody (CF®594)

Purpose: PLK1 (Marker of Mitosis)(AZ44), CF594 conjugate

Characteristics: Plk (for polo-like kinase) encodes a serine/threonine kinase that is closely related to polo and CDC5, genes that are required for passage through mitosis in Drosophila and Saccharomyces, respectively. Polo and Cdc5 both code for proteins that are involved in regulating the function of the mitotic spindle. Plk protein accumulates in the cell during the S and G2 phases of the cell cycle, Plk protein content and catalytic activity peak at the onset of mitosis, followed by a rapid reduction after mitosis. Plk expression is detectable in mitotically active tissues such as colon and placenta, as well as in tumors of various origins. It has also been suggested that Plk may serve as a marker of cell proliferation. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS. Useful for confirming that extracts have maintained CSF status and have not yet been activated into interphase. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length PLK1 Xenopus laevis protein

Isotype: IgG1, kappa

Application Details

Application Notes: Does not react with Human or Mouse, ELISA: For coating, order antibody without BSA, Western blot 1-2 μg/mL, Optimal dilution for a specific application should be determined by user.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Comment:

Positive Control: Mitotic CSF Xenopus egg extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: PLK1

Background:

Synonyms: Cell cycle regulated protein kinase, Polo like kinase 1 (PLK1), Serine/threonine-protein kinase PLK1, STPK13

Gene Symbol: plk1.S

Molecular Weight: 66 kDa

Gene ID: 380481

UniProt: P70032

Pathways: Cell Division Cycle, M Phase