RPS6KB2 antibody (pThr228)

Catalog No. ABIN732608

The Rabbit Polyclonal anti-RPS6KB2 antibody has been validated for WB, ELISA, IF (cc), IF (p), IHC (fro) and ICC. It is suitable to detect RPS6KB2 in samples from Human.

Quick Overview for RPS6KB2 antibody (pThr228) (ABIN732608)

Target: RPS6KB2 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 2 (RPS6KB2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS6KB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Product Details anti-RPS6KB2 Antibody

Binding Specificity: pThr228

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human P70 S6 Kinase beta around the phosphorylation site of Thr228

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for RPS6KB2

Target: RPS6KB2 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 2 (RPS6KB2))

Alternative Name: P70 S6 Kinase beta

Background:

Synonyms: Ribosomal protein S6 kinase beta-2, S6K-beta-2, S6K2, 70 kDa ribosomal protein S6 kinase 2, P70S6K2, p70-S6K 2, S6 kinase-related kinase, SRK, Serine/threonine-protein kinase 14B, p70 ribosomal S6 kinase beta, S6K-beta, p70 S6 kinase beta, p70 S6K-beta, p70 S6KB, p70-beta, RPS6KB2, STK14B

Background: This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013].

Gene ID: 6199

UniProt: Q9UBS0

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway