Retinoblastoma 1 antibody (CF®740)

Catalog No. ABIN7659621

This anti-Retinoblastoma 1 antibody is a Mouse Monoclonal antibody detecting Retinoblastoma 1 in IHC and IHC (fp). Suitable for Human and Mouse.

Quick Overview for Retinoblastoma 1 antibody (CF®740) (ABIN7659621)

Target: Retinoblastoma 1 (RB1)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Retinoblastoma 1 antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: 1F8

Product Details anti-Retinoblastoma 1 Antibody (CF®740)

Purpose: Rb1 (Tumor Suppressor Protein) (1F8), CF740 conjugate

Characteristics: This antibody recognizes a 105 kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. Its epitope is localized between aa 703-772. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Rb1 protein fragment of 283 amino acid residues

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1 μg/mL. Immunohistology (formalin) 1-2 μg/mL. Western blotting 0.5-1 μg/mL Immunoprecipitation 0.5-1 μg/500 μg protein lysate. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HeLa, A431 or BT-20 cells. Breast or Colon carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Alternative Name: Rb1

Background:

Synonyms: OSRC, Osteosarcoma, p105-Rb, PP105, pp110, pRb, Prepro retinoblastoma associated protein, RB1, Retinoblastoma 1, Retinoblastoma-associated protein

Gene Symbol: RB1

Molecular Weight: 105 kDa

Gene ID: 5925

UniProt: P06400

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy