CD15 antibody (AA 251-295)

Catalog No. ABIN733193

The Rabbit Polyclonal anti-CD15 antibody has been validated for FACS, WB, IHC (p), ELISA, IHC (fro), IF (p) and IF (cc). It is suitable to detect CD15 in samples from Human, Mouse and Rat. There are 2+ publications available.

Quick Overview for CD15 antibody (AA 251-295) (ABIN733193)

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD15 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-CD15 Antibody

Binding Specificity: AA 251-295

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse CD15

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CD15 antibody (ABIN733193)

Cong, Cao, Liu: "Effects of different feeder layers on culture of bovine embryonic stem cell-like cells in vitro." in: Cytotechnology, (2014) (PubMed).

Zschemisch, Eisenblätter, Rudolph, Glage, Dorsch: "Immortalized tumor derived rat fibroblasts as feeder cells facilitate the cultivation of male embryonic stem cells from the rat strain WKY/Ztm." in: SpringerPlus, Vol. 3, pp. 588, (2014) (PubMed).

Target Details for CD15

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Alternative Name: CD15/Fut4/SSEA-1

Background:

Synonyms: FAL, LeX, CD15, Ssea1, SSEA-1, FucT-IV, AI451562, Alpha-(1,3)-fucosyltransferase 4, Fucosyltransferase 4, Fucosyltransferase IV, Fuc-TIV, Galactoside 3-L-fucosyltransferase, Fut4, Elft

Background: May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.

Gene ID: 14345

UniProt: Q11127