TNF alpha antibody (PE,Cy7)

Catalog No. ABIN7670269

The Rat Monoclonal anti-TNF alpha antibody has been validated for ICFC. It is suitable to detect TNF alpha in samples from Mouse.

Quick Overview for TNF alpha antibody (PE,Cy7) (ABIN7670269)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is conjugated to PE,Cy7

Application: Intracellular Flow Cytometry (ICFC)

Clone: XT3-11

Product Details anti-TNF alpha Antibody (PE,Cy7)

Purpose: PE/Cyanine7 Anti-Mouse TNFα Antibody[XT3.11]

Immunogen: E-AB-F09822H

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 5 μL/tests

Buffer: Phosphate buffered solution, pH 7.2, containing 0.09 % stabilizer and 1 % protein protectant

Handling Advice: Please protected from prolonged exposure to light and do not freeze.

Storage: 4 °C

Storage Comment: This product can be stored at 2-8°C for 12 months.

Expiry Date: 12 months

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF-alpha

Background: Tumor necrosis factor-α,Cachectin,Necrosin,Macrophage cytotoxic factor,Differentiation inducing factor,TNFSF-2,TNF-a,TNF-alpha,TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is a 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD. TNF-α forms multimeric complexes, stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorrhagic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

UniProt: P06804

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome