Recombinant His Tag antibody

Catalog No. ABIN7670359

The Rabbit Monoclonal anti-His Tag antibody is suitable to detect His Tag. It has been validated for WB, ELISA, IP and ICC.

Quick Overview for Recombinant His Tag antibody (ABIN7670359)

Target: His Tag

Antibody Type: Recombinant Antibody

Host: Rabbit

Clonality: Monoclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: RM146X

Product Details anti-His Tag Antibody

Purpose: Rabbit monoclonal to His-Tag at either the N-Terminus (amino) or C-Terminus (carboxy)

Specificity: This antibody reacts to recombinant proteins containing the His-Tag fused to either the amino or carboxy terminus.

Cross-Reactivity (Details): Very low non-specific reactivity with endogenous proteins in mammalian or bacteria cells, compared with other anti-HisTag antibodies.

Purification: Protein A affinity purified from an animal origin-free culture supernatant

Immunogen: 6xHis-Tag coupled to KLH

Isotype: IgG

Application Details

Application Notes: WB: 0.01 μg/mL - 0.1 μg/mL, ELISA: 0.01 μg/mL - 0.2 μg/mL, IP: 0.5 μg/mL - 2 μg/mL, ICC: 0.5 μg/mL - 2 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 50 % Glycerol/PBS with 1 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20.0°C

Target Details for His Tag

Target: His Tag

Alternative Name: His-Tag

Target Type: Tag