CD47 antibody (PE-Cy5.5)

Catalog No. ABIN7673952

The Mouse Monoclonal anti-CD47 antibody has been validated for FACS. It is suitable to detect CD47 in samples from Human, Mouse and Rat.

Quick Overview for CD47 antibody (PE-Cy5.5) (ABIN7673952)

Target: CD47

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD47 antibody is conjugated to PE-Cy5.5

Application: Flow Cytometry (FACS)

Clone: MIAP410

Product Details anti-CD47 Antibody (PE-Cy5.5)

Purpose: PE-Cy5.5 Anti-Human/Mouse/Rat CD47 Antibody

Purification: 0

Purity: 0

Isotype: IgG1, kappa

Application Details

Application Notes: Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 μL staining volume or per 100 μL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0. 1 % sodium azide, 1 % BSA, pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: 4 °C

Storage Comment: 2-8°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for CD47

Target: CD47

Alternative Name: CD47

Background:

Synonyms: Cd47|IAP|Integrin-associated protein|Leukocyte surface antigen CD47

Background: CD47, also known as Integrin-Associated Protein (IAP), is a membrane protein of about 50 kD with an IgV-like extracelluluar domain, a five membrane-spanning segment and a short terminal cytoplasmic region. It is widely expressed on many cell types and often associated with beta 3 integrins. It has been reported that CD47 functions as a self marker. Red cells lacking CD47 were rapidly cleared from the bloodstream by splenic macrophages. By binding to SIRPα, CD47 controls hemostatic innate immune functions, such as phagocytosis and cell trafficking.

Molecular Weight: 0

UniProt: Q08722, Q61735, P97829