CD15 antibody

Catalog No. ABIN7674791

This anti-CD15 antibody is a Mouse Monoclonal antibody detecting CD15 in FACS, IHC, IHC (p) and D. Suitable for Xenopus laevis.

Quick Overview for CD15 antibody (ABIN7674791)

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Reactivity: Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD15 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Detection (D)

Clone: BRA4F1

Product Details anti-CD15 Antibody

Purpose: The antibody BRA4F1 is directed against human CD15. The antibody does not cross react with the sialylated form of CD15 (CD15s).

Specificity: The antibody BRA4F1 is directed against human CD15. The antibody does not cross react with the sialylated form of CD15 (CD15s).

Characteristics: CD15 is a carbohydrate that is referred to as the Lewis X antigen, also known as alpha-(1,3)-fucosyltransferase 4, which is expressed on a variety of glycolipids and glycoproteins found on the cell surface of neutrophils, monocytes, eosinophils and non-hematopoietic tissues including embryonic tissues. CD15 is reported to function as an adhesion molecule and is involved in chemotaxis and phagocytosis. Expression of CD15 is also associated with a wide variety of tumour cells including some adenocarcinomas and certain types of myeloid and lymphoid leukaemias. Reed-Sternberg cells, found in cases of Hodgkins Lymphoma, also express CD15 and antibodies recognising the CD15 antigen are often used to help differentiate between Hodgkins and non-Hodgkins lymphoma.

Purification: Purified

Immunogen: BRA4F1 is a mouse IgM monoclonal antibody derived from the fusion of SP2.0-Ag14 mouse myeloma cells with splenocytes from a BALB/c mouse immunized with K562 cells.

Isotype: IgM

Application Details

Application Notes: The BRA4F1 antibody is suitable for the detection of CD15 by flow cytometry, immunocytochemisty and immunohistochemistry of frozen and paraffin embedded tissues. Optimal antibody dilutions for the different applications should be determined by titration.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Each vial contains 100 μL 1 mg/mL purified monoclonal antibody in PBS containing 0.09 % sodium azide. The product is delivered in lyophilised format.

Buffer: Each vial contains 100 μL 1 mg/mL purified monoclonal antibody in PBS containing 0.09 % sodium azide. The product is delivered in lyophilised format.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Target Details for CD15

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Alternative Name: CD15