Recombinant EGFR antibody (C-Term)

Catalog No. ABIN7795642

The Rabbit Monoclonal anti-EGFR antibody (Clone BLR252L) (ABIN7795642) specifically detects EGFR in WB, IHC, IP and ICC. The antibody is reactive with Human and Mouse samples.

Quick Overview for Recombinant EGFR antibody (C-Term) (ABIN7795642)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This EGFR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: BLR252L

Product Details anti-EGFR Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-EGFR Recombinant Monoclonal Antibody [BLR252L]

Purification: Purified

Immunogen: Between 1160 and C-terminus

Isotype: IgG

Application Details

Application Notes: Flow-Cyt,Fixed in 4 % formaldehyde and permeabilized with 90 % methanol. 0.25 μL per 1 x 10^6 cells.,ICC,1100 to 1500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.,IHC,1100 to 1500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.,IP,6 μL/mg lysate ,WB,11000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1000 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09 % Sodium Azide, BSA-Free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2 - 8 °C

Expiry Date: 12 months

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: EGFR

Background: The epidermal growth factor receptor (EGFR) is a membrane spanning tyrosine kinase receptor for epidermal growth factor (EGF) and other ligands of the EGF family such as TGF-alpha, amphiregulin, betacellulin, epiregulin and heparin-binding EGF-like growth factor. Upon ligand binding, the EGFR homodimerizes or heterodimerizes with other ErbB receptor family members and tranduces a downstream signal via the ras-raf-mitogen activated protein kinase pathway or the phosphatidylinositol 3-kinase (PI3K)/Akt pathway. Signals from the EGFR function to influence cell proliferation, differentiation, and development.

Gene ID: 1956

UniProt: P00533

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins