Recombinant Smooth Muscle Actin antibody

Catalog No. ABIN7797464

This Rabbit Monoclonal antibody specifically detects Smooth Muscle Actin in WB, FACS and IF (p). It exhibits reactivity toward Human.

Quick Overview for Recombinant Smooth Muscle Actin antibody (ABIN7797464)

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Smooth Muscle Actin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Grade: KD Validated

Clone: 23GB605

Product Details anti-Smooth Muscle Actin Antibody

Expression System: HEK-293 Cells

Purpose: KD-Validated alpha smooth muscle Actin Recombinant Rabbit mAb

Cross-Reactivity: Mouse, Rat

Purification: Affinity Purified

Immunogen: A synthesized peptide derived from human alpha smooth muscle Actin

Isotype: IgG

Application Details

Application Notes: Western Blotting (WB): 1:2,000-1:10,000, Flow Cytometry (FCM): 1:200-1:2,000, Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details for Smooth Muscle Actin

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Alternative Name: alpha smooth muscle Actin

Molecular Weight: 42 kDa

Gene ID: 59

UniProt: P62736

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development