Recombinant CHEK1 antibody (pSer280)

Catalog No. ABIN7798037

This Rabbit Monoclonal antibody specifically detects CHEK1 in WB and FACS. It exhibits reactivity toward Human.

Quick Overview for Recombinant CHEK1 antibody (pSer280) (ABIN7798037)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CHEK1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Grade: KD Validated

Clone: 25GB3390

Product Details anti-CHEK1 Antibody

Binding Specificity: pSer280

Expression System: HEK-293 Cells

Purpose: KD-Validated Phospho-Chk1 (S280) Recombinant Rabbit mAb

Purification: Affinity Purified

Immunogen: A synthesized peptide derived from human Phospho-Chk1 (S280)

Isotype: IgG

Application Details

Application Notes: Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: Chk1

Molecular Weight: 54 kDa

Gene ID: 1111

UniProt: O14757

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair