Recombinant POLB antibody

Catalog No. ABIN7799875

The Rabbit Monoclonal anti-POLB antibody (Clone 24GB1475) (ABIN7799875) specifically detects POLB in WB, ICC and FACS. The antibody is reactive with Human samples.

Quick Overview for Recombinant POLB antibody (ABIN7799875)

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This POLB antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Grade: KD Validated

Clone: 24GB1475

Product Details anti-POLB Antibody

Expression System: HEK-293 Cells

Purpose: KD-Validated DNA Polymerase beta Recombinant Rabbit mAb

Cross-Reactivity: Mouse, Rat

Purification: Affinity Purified

Immunogen: A synthesized peptide derived from human DNA polymerase beta

Isotype: IgG

Application Details

Application Notes: Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000, Immunocytochemistry (ICC): 1:100-1:1,000

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS (pH 7.4) containing 50% glycerol and 0.02% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details for POLB

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Alternative Name: DNA Polymerase beta

Molecular Weight: 38 kDa

Gene ID: 5423

UniProt: P06746

Pathways: DNA Damage Repair