Topoisomerase II alpha antibody

Catalog No. ABIN7800573

The Rabbit Polyclonal anti-Topoisomerase II alpha antibody is suitable to detect Topoisomerase II alpha in samples from Human. It has been validated for WB.

Quick Overview for Topoisomerase II alpha antibody (ABIN7800573)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB)

Grade: KD Validated

Product Details anti-Topoisomerase II alpha Antibody

Purpose: KD-Validated TOP2A Rabbit pAb

Cross-Reactivity: Rat

Purification: Affinity Purified

Immunogen: A synthesized peptide derived from human Topoisomerase 2 alpha

Isotype: IgG

Application Details

Application Notes: Western Blotting (WB): 1:500-1:2,500

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS (pH 7.4) containing 50% glycerol and 0.02% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: TOP2A

Molecular Weight: 174 kDa

Gene ID: 7153

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases