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Recombinant Vimentin antibody (pSer72)

Name: Recombinant Vimentin antibody (pSer72)
Brand: antibodies-online
SKU: ABIN7800835
Availability: InStock

The Rabbit Monoclonal anti-Vimentin antibody (Clone 25GB3480) (ABIN7800835) specifically detects Vimentin in WB, FACS and IF (p). The antibody is reactive with Human samples.

Western blotting analysis using phospho-vimentin (Ser72) antibody (ABIN7800835). (Recombinant Vimentin antibody (pSer72))

Immunohistochemistry was performed on paraffin-embedded human endometrial carcinoma using phospho-vimentin (Ser72) antibody (ABIN7800835), 1:100). (Recombinant Vimentin antibody (pSer72))

IHC (p)

Flow cytometric analysis of Phospho-Vimentin (Ser72) expression in HT- cells using Phospho-Vimentin (Ser72) antibody (ABIN7800835), 1:2,000). (Recombinant Vimentin antibody (pSer72))

FACS

4 images

Catalog No. ABIN7800835

$598.92

Plus shipping costs $50.00

100 μL

Shipping to: United States

Delivery in 3 to 5 Business Days

Quick Overview for Recombinant Vimentin antibody (pSer72) (ABIN7800835)

Target

See all Vimentin (VIM) Antibodies

Vimentin (VIM)

Antibody Type

Recombinant Antibody

Reactivity

Human

Host

Rabbit

Clonality

Monoclonal

Conjugate

This Vimentin antibody is un-conjugated

Application

Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Grade

KD Validated

Clone

25GB3480

Discover our top product VIM Primary Antibody

Binding Specificity
32

31

17

16

12

12

11

7

6

5

4

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer72

Expression System

HEK-293 Cells

Purpose

KD-Validated Phospho-Vimentin (Ser72) Recombinant Rabbit mAb

Cross-Reactivity

Mouse, Rat

Purification

Affinity Purified

Immunogen

A synthesized peptide derived from human Phospho-Vimentin (Ser72)

Isotype

IgG
anti-Vimentin (VIM) (AA 371-466) antibody
VIM Reactivity: Human, Rat, Mouse, Pig, Cow, Monkey WB, ELISA, FACS, IHC (p), ICC, IF (p), IHC (fro), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody
VIM Reactivity: Human, Rat, Mouse WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reactivity: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Rat, Mouse, Pig, Chicken WB, IHC (p), ICC, IP Host: Mouse Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, IF Host: Mouse Monoclonal C12 unconjugated

anti-Vimentin (VIM) (AA 41-140) antibody
VIM Reactivity: Human WB, ELISA, IF, IP Host: Mouse Monoclonal 3E9 unconjugated

Application Notes

Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000, Immunohistochemistry-Paraffin (IHC-P): 1:50-1:100

Comment

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions

For Research Use only
Format

Liquid

Buffer

PBS (pH 7.4) containing 50% glycerol and 0.02% sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Store at -20 °C for one year.

Expiry Date

12 months
Target

Vimentin (VIM)

Alternative Name

Vimentin

Molecular Weight

54 kDa

Gene ID

7431

UniProt

P08670

Pathways

Caspase Cascade in Apoptosis