MAP3K12 antibody (C-Term)

Catalog No. ABIN7824556

This Mouse Monoclonal antibody specifically detects MAP3K12 in ELISA, WB, IHC and ICC. It exhibits reactivity toward Mouse.

Quick Overview for MAP3K12 antibody (C-Term) (ABIN7824556)

Target: MAP3K12 (Mitogen-Activated Protein Kinase Kinase Kinase 12 (MAP3K12))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAP3K12 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Grade: KO Validated

Clone: N377-20

Product Details anti-MAP3K12 Antibody

Binding Specificity: AA 727-888, C-Term

Purpose: Anti-MAP3K12 Antibody

Specificity: No off-targets reported for MAP3K13

Cross-Reactivity: Rat

Characteristics: Our Anti-MAP3K12 mouse monoclonal primary antibody is produced in-house from hybridoma clone N377/20. It is KO validated, detects mouse and rat MAP3K12, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Purity: Purified mAbs are >90 % specific antibody.

Immunogen: Fusion protein amino acids 727-888 (C-terminus) of mouse MAP3K12 (accession number Q60700) produced recombinantly in E. Coli

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 10 mM Tris, 50 mM Sodium Chloride, 0.065 % Sodium Azide 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: For maximum recovery of product, centrifuge the vial prior to removing the cap.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C.

Expiry Date: 24 months

Target Details for MAP3K12

Target: MAP3K12 (Mitogen-Activated Protein Kinase Kinase Kinase 12 (MAP3K12))

Alternative Name: MAP3K12

Background:

Synonyms: Mitogen-activated protein kinase kinase kinase 12 (EC 2.7.11.25) (Dual leucine zipper bearing kinase) (DLK) (Leucine-zipper protein kinase) (ZPK) (MAPK-upstream kinase) (MUK) (Mixed lineage kinase)

Description Logic: Our Anti-MAP3K12 mouse monoclonal primary antibody is produced from hybridoma clone N377/20. It is KO validated, detects mouse and rat MAP3K12, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.

Gene Name Alternatives: Map3k12 Zpk

Molecular Weight: 120 kDa

UniProt: Q60700

Pathways: MAPK Signaling