ERCC5 antibody (AA 115-964)

Catalog No. ABIN7825344

This Rabbit Polyclonal antibody specifically detects ERCC5 in WB, ELISA, IHC, IF, ICC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ERCC5 antibody (AA 115-964) (ABIN7825344)

Target: ERCC5 (DNA Repair Protein Complementing XP-G Cells (ERCC5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERCC5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-ERCC5 Antibody

Binding Specificity: AA 115-964

Purpose: Anti-XPG/ERCC5 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-XPG/ERCC5 Antibody catalog # A01770-2. Tested in WB, IHC, IF, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human XPG/ERCC5 recombinant protein (Position: K115-R964). Human XPG/ERCC5 shares 66.6% amino acid (aa) sequence identity with mouse XPG/ERCC5.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ERCC5

Target: ERCC5 (DNA Repair Protein Complementing XP-G Cells (ERCC5))

Alternative Name: ERCC5

Background: This gene encodes a single-strand specific DNA endonuclease that makes the 3' incision in DNA excision repair following UV-induced damage. The protein may also function in other cellular processes, including RNA polymerase II transcription, and transcription-coupled DNA repair. Mutations in this gene cause xeroderma pigmentosum complementation group G (XP-G), which is also referred to as xeroderma pigmentosum VII (XP7), a skin disorder characterized by hypersensitivity to UV light and increased susceptibility for skin cancer development following UV exposure. Some patients also develop Cockayne syndrome, which is characterized by severe growth defects, cognitive disability, and cachexia. Read-through transcription exists between this gene and the neighboring upstream BIVM (basic, immunoglobulin-like variable motif containing) gene.

Molecular Weight: 200 kDa

Gene ID: 2073

UniProt: P28715

Pathways: DNA Damage Repair