HK3 antibody (AA 1-789)

Catalog No. ABIN7825455

This Rabbit Polyclonal antibody specifically detects HK3 in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for HK3 antibody (AA 1-789) (ABIN7825455)

Target: HK3 (Hexokinase 3 (White Cell) (HK3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-HK3 Antibody

Binding Specificity: AA 1-789

Purpose: Anti-HK3 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-HK3 Antibody catalog # A05145-3. Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HK3 recombinant protein (Position: M1-K789). Human HK3shares 84.7% and 84.8% amino acid (aa) sequence identity with mouse and rat HK3, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for HK3

Target: HK3 (Hexokinase 3 (White Cell) (HK3))

Alternative Name: HK3

Background: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate.

Molecular Weight: 99 kDa

Gene ID: 3101

UniProt: P52790

Pathways: Carbohydrate Homeostasis, Warburg Effect