Recombinant Insulin antibody

Catalog No. ABIN7826125

This Rabbit Monoclonal antibody specifically detects Insulin in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant Insulin antibody (ABIN7826125)

Target: Insulin (INS)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Insulin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: HMV308

Product Details anti-Insulin Antibody

Purpose: Insulin antibody (HMV308)

Immunogen: Recombinant human INS fragment

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Pancreas: A strong Insulin staining should be seen in a large fraction of islet cells.

Product Description/Background: Insulin antibody validated for immunohistochemistry on 76 different Normal Tissues

Negative Control: Colon: Insulin staining must be absent in all cell types.

Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.

Expiry Date: 24 months

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: Insulin

Background: Gene Alias: Insulin, IDDM , IDDM1 , IDDM2 , ILPR , IRDN , MODY10

UniProt: P01308

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation