Recombinant TIGIT antibody

Catalog No. ABIN7826145

The Rabbit Monoclonal anti-TIGIT antibody (Clone HMV322) (ABIN7826145) specifically detects TIGIT in IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant TIGIT antibody (ABIN7826145)

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This TIGIT antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: HMV322

Product Details anti-TIGIT Antibody

Purpose: TIGIT antibody (HMV322)

Immunogen: Recombinant human TIGIT fragment

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Tonsil: A moderate to strong TIGIT staining should be seen in a subset of germinal centre cells (CD4+ follicular T helper cells located in the germinal centre periphery orientated towards the tonsil surface epithelium). Subsets of other lymphocytes should also be positive but less intense.

Product Description/Background: TIGIT antibody validated for immunohistochemistry on 76 different Normal Tissues

Negative Control: Tonsil: TIGIT staining should be absent in all epithelial cells and in most lymphocytes.

Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.

Expiry Date: 24 months

Target Details for TIGIT

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Alternative Name: TIGIT

Background: Gene Alias: T cell immunoreceptor with Ig and ITIM domains , VSIG9 , VSTM3 , WUCAM

UniProt: Q495A1

Pathways: Cancer Immune Checkpoints