Recombinant TIGIT antibody
Quick Overview for Recombinant TIGIT antibody (ABIN7826146)
Target
See all TIGIT AntibodiesAntibody Type
Reactivity
Host
Clonality
Conjugate
Application
Clone
-
-
Purpose
- TIGIT antibody (HMV323)
-
Immunogen
- Recombinant human TIGIT fragment
-
Isotype
- IgG
-
-
-
-
Application Notes
- IHC 1:100-1:200
-
Comment
-
Positive Control: Tonsil: A moderate to strong TIGIT staining should be seen in a subset of germinal centre cells (CD4+ follicular T helper cells located in the germinal centre periphery orientated towards the tonsil surface epithelium). Subsets of other lymphocytes should also be positive but less intense.
Product Description/Background: TIGIT antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Tonsil: TIGIT staining should be absent in all epithelial cells and in most lymphocytes.
-
Protocol
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Storage
- 4 °C,-20 °C,-80 °C
-
Storage Comment
- Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
-
Expiry Date
- 24 months
-
-
- TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))
-
Alternative Name
- TIGIT
-
Background
- Gene Alias: T cell immunoreceptor with Ig and ITIM domains , VSIG9 , VSTM3 , WUCAM
-
UniProt
- Q495A1
-
Pathways
- Cancer Immune Checkpoints
Target
-