mCherry antibody

Catalog No. ABIN7830291

The Goat Polyclonal anti-mCherry antibody (ABIN7830291) specifically detects mCherry in WB, IF, IHC (fro), IHC (p) and EM. The antibody is reactive with Discosoma and Tomato samples.

Quick Overview for mCherry antibody (ABIN7830291)

Target: mCherry (mCherry Fluorescent Protein)

Reactivity: Discosoma, Tomato

Host: Goat

Clonality: Polyclonal

Conjugate: This mCherry antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Electron Microscopy (EM)

Product Details anti-mCherry Antibody

Purpose: anti-mCherry

Sequence: MVSKGEEDNM AIIKEFMRFK VHMEGSVNGH EFEIEGEGEG RPYEGTQTAK LKVTKGGPLP FAWDILSPQF MYGSKAYVKH PADIPDYLKL SFPEGFKWER VMNFEDGGVV TVTQDSSLQD GEFIYKVKLR GTNFPSDGPV MQKKTMGWEA SSERMYPEDG ALKGEIKQRL KLKDGGHYDA EVKTTYKAKK PVQLPGAYNV NIKLDITSHN EDYTIVEQYE RAEGRHSTGG MDELYK

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein).

Characteristics: Red Fluorescent Protein

Purification: Epitope affinity purified

Immunogen: Purified recombinant peptide produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Restrictions: For Research Use only

Handling

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Target Details for mCherry

Target: mCherry (mCherry Fluorescent Protein)

Alternative Name: mCherry

Background:

Goat polyclonal antibody to mCherry (Cherry fluorescent protein). mCherry protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. mCherry is a ~27 kDa protein that is optimally excited at a 587 nm and has a maximum of emission at 610 nm. It is a basic (constitutively fluorescent) red fluorescent protein and It is reported to be a very rapidly-maturing monomer with low acid sensitivity.

red fluorescent protein antibody.

Molecular Weight: 27 kDa