Recombinant GFP antibody (AA 1-238)
Quick Overview for Recombinant GFP antibody (AA 1-238) (ABIN7830377)
Target
See all GFP AntibodiesAntibody Type
Reactivity
Host
Clonality
Conjugate
Application
Grade
Clone
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Binding Specificity
- AA 1-238
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Expression System
- Mammalian cells
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Purpose
- Anti-GFP (N86/38) Recombinant Chicken Chimeric mAb (BSA and Azide free)
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Cross-Reactivity (Details)
- Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively)Does not cross-react with Red fluorescent protein (dsRed)
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Characteristics
- This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N86/38 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N86/38 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and buffer exchanged into 1X PBS.
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Purification
- Purified by affinity chromatography
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Immunogen
- Fusion protein amino acids 1-238 (full-length) of jellyfish green fluorescent protein (accession number P42212) produced recombinantly in E. Coli
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Isotype
- IgY
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Application Notes
- 1:500-1:3000
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- PBS pH 7.4
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Preservative
- Azide free
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Storage
- 4 °C,-20 °C
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Storage Comment
- Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
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Expiry Date
- 24 months
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- GFP (Green Fluorescent Protein (GFP))
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Alternative Name
- GFP
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Background
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Green fluorescent protein
Our chicken Anti-GFP carrier-free recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N86/38. It detects n/a GFP and is purified by affinity chromatography. It works in WB, IHC, ICC, ELISA.
GFP
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Molecular Weight
- 27 kDa
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UniProt
- P42212
Target
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