CRISPR-Cas9 (N-Term) antibody

Catalog No. ABIN7833199

The Mouse Monoclonal anti-CRISPR-Cas9 antibody is suitable to detect CRISPR-Cas9 in samples from Streptococcus pyogenes. It has been validated for WB, IF and IP.

Quick Overview for CRISPR-Cas9 (N-Term) antibody (ABIN7833199)

Target: CRISPR-Cas9

Reactivity: Streptococcus pyogenes

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details

Binding Specificity: N-Term

Purpose: Anti-Cas9 | CRISPR-associated endonuclease 9 (monoclonal)

Cross-Reactivity (Details): Cas9 from Streptococcus pyogenes

Purification: Protein A purified

Immunogen: Recombinant protein part from the N-terminus of Cas9 from Streptococcus pyogenes.

Isotype: IgG1 kappa

Application Details

Application Notes: 1 : 200 (IF), (IP), 1 : 1000-1 : 5000 (WB)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Total IgG. Protein A purified in PBS. Contains 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C,-80 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. For long term storage -80°C is recommended. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details

Target: CRISPR-Cas9

Alternative Name: Cas9

Background: Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA. Alternative name: Csn1.

Molecular Weight: Depends upon a MW of a protein which is a fusion partner