Integrin Alpha2b antibody

Catalog No. ABIN7836291

This Mouse Monoclonal antibody specifically detects Integrin Alpha2b in FACS and IF. It exhibits reactivity toward Human.

Quick Overview for Integrin Alpha2b antibody (ABIN7836291)

Target: Integrin Alpha2b (CD41)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin Alpha2b antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: P256-1

Product Details anti-Integrin Alpha2b Antibody

Purpose: Mouse monoclonal antibody to CD41

Specificity: Recognizes human CD41

Purification: The antibody was purified by affinity chromatography.

Application Details

Application Notes: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Target Details for Integrin Alpha2b

Target: Integrin Alpha2b (CD41)

Alternative Name: ITGA2B

Background: GP2B, ITGAB, Integrin alpha-IIb, GPalpha IIb, GPIIb, Platelet membrane glycoprotein IIb, CD41

Gene ID: 3674

UniProt: P08514

Pathways: Integrin Complex