Integrin Alpha2b antibody (PE)

Catalog No. ABIN7836296

The PE-conjugated Mouse Monoclonal anti-Integrin Alpha2b antibody (Clone HIP8) (ABIN7836296) specifically detects Integrin Alpha2b in FACS and IF. The antibody is reactive with Human samples.

Quick Overview for Integrin Alpha2b antibody (PE) (ABIN7836296)

Target: Integrin Alpha2b (CD41)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin Alpha2b antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: HIP8

Product Details anti-Integrin Alpha2b Antibody (PE)

Purpose: Mouse monoclonal antibody PE labled to CD41

Specificity: Recognizes human CD41

Purification: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD41.

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for Integrin Alpha2b

Target: Integrin Alpha2b (CD41)

Alternative Name: ITGA2B

Background: GP2B, ITGAB, Integrin alpha-IIb, GPalpha IIb, GPIIb, Platelet membrane glycoprotein IIb, CD41

Gene ID: 3674

UniProt: P08514

Pathways: Integrin Complex