CD55 antibody (PE-Cy5)

Catalog No. ABIN7836469

The Mouse Monoclonal anti-CD55 antibody is suitable to detect CD55 in samples from Human. It has been validated for FACS and IF.

Quick Overview for CD55 antibody (PE-Cy5) (ABIN7836469)

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD55 antibody is conjugated to PE-Cy5

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 143-30

Product Details anti-CD55 Antibody (PE-Cy5)

Purpose: Mouse monoclonal antibody PE/Cy5 labled to CD55

Specificity: Recognizes human CD55

Purification: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD55.

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: CD55

Background: CR, DAF, Complement decay-accelerating factor, CD antigen CD55

Gene ID: 1604

UniProt: P08174

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity