ERK2 antibody (pThr185, pThr202)

Catalog No. ABIN7838214

This Rabbit Monoclonal antibody specifically detects ERK2 in WB and IP. It exhibits reactivity toward Human, Rat and Hamster.

Quick Overview for ERK2 antibody (pThr185, pThr202) (ABIN7838214)

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Reactivity: Human, Rat, Hamster

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ERK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-ERK2 Antibody

Binding Specificity: pThr185, pThr202

Purpose: Rabbit monoclonal antibody to ERK1/2 (Phospho-T202/T185)

Specificity: Recognizes endogenous levels of ERK1/2 protein only when phosphorylated at T202/T185.

Purification: The antibody was purified by immunogen affinity chromatography.

Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T202/T185 of human ERK1/2 protein. The exact sequence is proprietary.

Application Details

Application Notes: WB (1/500 - 1/1000), IP (1/10 - 1/50)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in 50 mM Tris-Glycine ( pH 7.4), 0.15M NaCl, 50 % Glycerol, 0.01 % Sodium azide and 0.05 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Target Details for ERK2

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Alternative Name: MAPK1

Background: MAPK3, ERK1, PRKM3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubule-associated protein 2 kinase, p44-ERK1, MAPK1, ERK2, PRKM1, PRKM2, Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2, ERK-2, MAP kinase isoform p42, p42-MAPK, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2

Gene ID: 5595, 50689

UniProt: P27361

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins