ERK2 antibody (pThr185, pThr202)

Catalog No. ABIN7838215

The Rabbit Monoclonal anti-ERK2 antibody is suitable to detect ERK2 in samples from Human and Rat. It has been validated for WB and IP.

Quick Overview for ERK2 antibody (pThr185, pThr202) (ABIN7838215)

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ERK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-ERK2 Antibody

Binding Specificity: pThr185, pThr202

Purpose: Rabbit monoclonal antibody to ERK1/2 (Phospho-T202/T185)

Specificity: Recognizes endogenous levels of ERK1/2 protein only when phosphorylated at T202/T185.

Purification: The antibody was purified by immunogen affinity chromatography.

Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T202/T185 of human ERK1/2 protein. The exact sequence is proprietary.

Application Details

Application Notes: WB (1/500 - 1/1000), IP (1/10 - 1/50)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in 50 mM Tris-Glycine ( pH 7.4), 0.15M NaCl, 50 % Glycerol, 0.01 % Sodium azide and 0.05 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Target Details for ERK2

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Alternative Name: MAPK1

Background: MAPK3, ERK1, PRKM3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubule-associated protein 2 kinase, p44-ERK1, MAPK1, ERK2, PRKM1, PRKM2, Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2, ERK-2, MAP kinase isoform p42, p42-MAPK, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2

Gene ID: 5595, 50689

UniProt: P27361

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins