IFNAR1 antibody

Catalog No. ABIN7839936

This Mouse Monoclonal antibody specifically detects IFNAR1 in FACS and IF. It exhibits reactivity toward Mouse.

Quick Overview for IFNAR1 antibody (ABIN7839936)

Target: IFNAR1 (Interferon alpha/beta Receptor 1 (IFNAR1))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This IFNAR1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: MAR1-5A3

Product Details anti-IFNAR1 Antibody

Purpose: Mouse monoclonal antibody to IFNAR1

Specificity: Recognizes mouse IFNAR1

Purification: The antibody was purified by affinity chromatography.

Immunogen: Exogenously expressed murine IFNAR1 extracellular domain

Application Details

Application Notes: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Target Details for IFNAR1

Target: IFNAR1 (Interferon alpha/beta Receptor 1 (IFNAR1))

Alternative Name: IFNAR1

Background: IFNAR, Interferon alpha/beta receptor 1, IFN-R-1, IFN-alpha/beta receptor 1, Cytokine receptor class-II member 1, Cytokine receptor family 2 member 1, CRF2-1, Type I interferon receptor 1

Gene ID: 15975

Pathways: JAK-STAT Signaling, Hepatitis C