TIGIT antibody

Catalog No. ABIN7845436

The Mouse Monoclonal anti-TIGIT antibody is suitable to detect TIGIT in samples from Mouse. It has been validated for FACS and IF.

Quick Overview for TIGIT antibody (ABIN7845436)

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIGIT antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 1G9

Product Details anti-TIGIT Antibody

Purpose: Mouse monoclonal antibody to TIGIT

Specificity: Recognizes mouse TIGIT

Purification: The antibody was purified by affinity chromatography.

Immunogen: TIGIT knockout mice immunized with recombinant mouse TIGIT tetramers

Application Details

Application Notes: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Target Details for TIGIT

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Alternative Name: TIGIT

Background: VSIG9, VSTM3, T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain-containing protein 3

Gene ID: 100043314

Pathways: Cancer Immune Checkpoints