H2AFX antibody (pSer140)

Catalog No. ABIN742538

The Rabbit Polyclonal anti-H2AFX antibody has been validated for WB, ELISA, IHC (p), ICC, FACS, IF (p), IF (cc) and IHC (fro). It is suitable to detect H2AFX in samples from Human. There are 4+ publications available.

Quick Overview for H2AFX antibody (pSer140) (ABIN742538)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This H2AFX antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-H2AFX Antibody

Binding Specificity: pSer140

Specificity: This phosphorylation site is homologous to that of Ser140 in Mouse and Rat.

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Histone H2AX around the phosphorylation site of Ser140

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-H2AFX antibody (ABIN742538)

Chen, Song, Hou, Li: "Reactive oxygen species induced by icaritin promote DNA strand breaks and apoptosis in human cervical cancer cells." in: Oncology reports, Vol. 41, Issue 2, pp. 765-778, (2019) (PubMed).

Shi, Wang, Li, Bai, Li, Li, Wang, Zhou: "Dihydroartemisinin induces autophagy-dependent death in human tongue squamous cell carcinoma cells through DNA double-strand break-mediated oxidative stress." in: Oncotarget, Vol. 8, Issue 28, pp. 45981-45993, (2018) (PubMed).

Song, Shi, Liu, Wang, Wang, Song, Song: "Unpredicted Downregulation of RAD51 Suggests Genome Instability Induced by Tetrachlorobenzoquinone." in: Chemical research in toxicology, Vol. 29, Issue 12, pp. 2184-2193, (2016) (PubMed).

Shi, Yang, Mao, Wu, Ren, Yang, Yin, Wei, Li, Wang: "Nutlin-3-induced redistribution of chromatin-bound IFI16 in human hepatocellular carcinoma cells in vitro is associated with p53 activation." in: Acta pharmacologica Sinica, Vol. 36, Issue 2, pp. 252-8, (2015) (PubMed).

Target Details for H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Alternative Name: Histone H2a.X

Background:

Synonyms: H2AX, H2A.X, H2A/X, Histone H2AX, Histone H2A.X, H2AFX

Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Gene ID: 3014

UniProt: P16104

Pathways: Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus