Recombinant SOX10 antibody (AA 115-269)

Catalog No. ABIN7865459

The Mouse Monoclonal anti-SOX10 antibody is suitable to detect SOX10 in samples from Human and Mouse. It has been validated for WB and IHC.

Quick Overview for Recombinant SOX10 antibody (AA 115-269) (ABIN7865459)

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOX10 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RSOX10-13506

Product Details anti-SOX10 Antibody

Binding Specificity: AA 115-269

Expression System: CHO Cells

Purpose: Recombinant SOX10 (Melanoma Marker) Antibody

Specificity: The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a protein of ~55 kDa, identified as SOX10. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.

Purification: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogen: Recombinant human SOX10 protein fragment (around aa115-269) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for SOX10

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Alternative Name: SOX10

Background:

DOM, PCWH, SOX10, SRY (sex determining region Y) box 10, SRY box containing gene 10, SRY related HMG box gene 10, Waardenburg syndrome type 2E(WS2E), WS4, Waardenburg syndrome type 4C (WS4C)

UACC-257, UACC-62, COLO-38 or HepG2 cells. Human melanoma, glioma or skin.

Molecular Weight: 49-58kDa

Gene ID: 6663, 376984

UniProt: P56693

Pathways: Chromatin Binding