Recombinant Melanoma gp100 antibody (AA 561-661)

Catalog No. ABIN7857671

This Rabbit Monoclonal antibody specifically detects Melanoma gp100 in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant Melanoma gp100 antibody (AA 561-661) (ABIN7857671)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Melanoma gp100 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: PMEL-7554R

Product Details anti-Melanoma gp100 Antibody

Binding Specificity: AA 561-661

Expression System: CHO Cells

Purpose: Recombinant gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker) Antibody

Specificity: The gp100 molecule is a 100kDa glycosylated protein that is cleaved into a small (26kDa) carboxy-terminal fragment and a larger amino- terminal section (60 64 kDa), which is subsequently cleaved to generate 26kDa and 34 38kDa fragments. By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa).

Purification: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogen: Recombinant fragment (around aa 561-661) of human SILV protein (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Alternative Name: gp100

Background:

95 kDa melanocyte-specific secreted glycoprotein, M-beta, Melanocyte lineage specific antigen GP100, Melanocyte protein Pmel 17, Melanoma associated ME20 antigen, Melanosomal matrix protein17, Premelanosome protein (PMEL17), Secreted melanoma-associated ME20 antigen, SILV, Silver homolog

Skin: A moderate to strong cytoplasmic PMEL immunostaining should be seen in all melanocytes of the skin.

Molecular Weight: 26-100kDa

Gene ID: 6490, 95972

UniProt: P40967