Recombinant Tyrosinase-Related Protein 1 antibody (C-Term)

Catalog No. ABIN7865490

The Mouse Monoclonal anti-Tyrosinase-Related Protein 1 antibody (Clone rG3E6) (ABIN7865490) specifically detects Tyrosinase-Related Protein 1 in IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant Tyrosinase-Related Protein 1 antibody (C-Term) (ABIN7865490)

Target: Tyrosinase-Related Protein 1 (TYRP1)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Tyrosinase-Related Protein 1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: RG3E6

Product Details anti-Tyrosinase-Related Protein 1 Antibody

Binding Specificity: C-Term

Expression System: CHO Cells

Purpose: Recombinant Tyrosinase-Related Protein-1 (TYRP-1) (Melanoma Marker) Antibody

Specificity: Tyrosinase-related protein-1 (TRP-1) is a member of a family of proteins which are involved in melanin biosynthesis. The catalytic function of TRP-1 has not been fully resolved but the enzyme appears to be important in the oxidation of 5,6-dihydroxyindole-2-carboxylic acid to form a high molecular weight pigmented biopolymer. In mammals, there are two basic types of melanin, the brown-black eumelanin and the reddish-yellow phaeomelanin. The concentrations of each are variable and are not related to skin type. In skin exposed to suberythemal doses of UVB, an increase in the number of melanocytes expressing TRP-1 and TRP-2 was observed with no increase noted in the number of tyrosinase-expressing melanocytes. In normal, untreated skin the number of melanocytes which immunohistochemically stain for either TRP-1, TRP-2 or tyrosinase appears to be similar irrespective of skin type. TRP-1 is also expressed in more than 50 per cent of choroidal melanocytes of the adult eye.

Purification: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogen: Prokaryotic recombinant fusion protein corresponding to the C-terminus of the TRP-1 molecule

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Tyrosinase-Related Protein 1

Target: Tyrosinase-Related Protein 1 (TYRP1)

Alternative Name: Tyrosinase-Related Protein-1

Background:

5, 6 dihydroxyindole 2 carboxylic acid oxidase, 6-dihydroxyindole-2-carboxylic acid oxidase, Associated with iris pigmentation, CAS2, Catalase B (CATB), DHICA oxidase, Glycoprotein75 (GP75), Melanoma antigen gp75

SK-MEL-19, SK-MEL-23, SK-MEL-30 or SK-MEL-37 cells. Human Skin or Melanoma.

Molecular Weight: 75kDa

Gene ID: 7306, 270279

UniProt: P17643