Recombinant Cyclin A antibody (N-Term)

Catalog No. ABIN7857714

The Mouse Monoclonal anti-Cyclin A antibody is suitable to detect Cyclin A in samples from Human. It has been validated for WB, IHC, IF and FACS.

Quick Overview for Recombinant Cyclin A antibody (N-Term) (ABIN7857714)

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: R6E6

Product Details anti-Cyclin A Antibody

Binding Specificity: N-Term

Expression System: CHO Cells

Purpose: Recombinant Cyclin A2 / CCNA2 (Marker of Tumor Proliferation) Antibody

Specificity: It recognizes a protein of 54 kDa, which is identified as cyclin A. Its epitope is located amino acids 144-148 of human Cyclin A2. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase.

Purification: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogen: Recombinant protein corresponding to the N-terminal fragment of cyclin A protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Cyclin A

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Alternative Name: Cyclin A2

Background:

CCN1, CCNA, CCNA2, CCNA2_HUMAN, Cyclin A2

K-562 or HeLa cells. Human tonsil or lymph node. EL4.

Molecular Weight: 54kDa

Gene ID: 890, 58974

UniProt: P20248

Pathways: PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA