Recombinant Renal Cell Carcinoma (Gp200) (AA 80-100), (N-Term) antibody

Catalog No. ABIN7865169

This Rabbit Monoclonal antibody specifically detects Renal Cell Carcinoma (Gp200) in IHC and WB. It exhibits reactivity toward Human, Rat and Guinea Pig.

Quick Overview for Recombinant Renal Cell Carcinoma (Gp200) (AA 80-100), (N-Term) antibody (ABIN7865169)

Target: Renal Cell Carcinoma (Gp200)

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat, Guinea Pig

Host: Rabbit

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Clone: CA9-9621R

Product Details

Binding Specificity: AA 80-100, N-Term

Expression System: CHO Cells

Purpose: Recombinant Renal Cell Carcinoma (Carbonic Anhydrase IX) Antibody

Specificity: Recognizes a glycoprotein of ~200 kDa, identified as carbonic anhydrase IX (CAIX/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93 % of primary and 84 % of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

Purification: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogen: Extracellular N-terminal proteoglycan-like region. Epitope is between aa80-100.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details

Target: Renal Cell Carcinoma (Gp200)

Alternative Name: Renal Cell Carcinoma

Background:

CA-IX, CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, Carbonic anhydrase IX, Carbonic dehydratase, G250, Membrane antigen MN, P54/58N, pMW1, RCC-associated antigen G250, Renal cell carcinoma-associated antigen G250

HeLa cells. Normal kidney or Human Stomach, Rat Stomach or Guinea pig Stomach (WB) renal cell carcinoma (IHC).

Molecular Weight: 55kDa

Gene ID: 768, 63287

UniProt: Q16790