Renal Cell Carcinoma (Gp200) (AA 314-410) antibody

Catalog No. ABIN7863513

The Mouse Monoclonal anti-Renal Cell Carcinoma (Gp200) antibody is suitable to detect Renal Cell Carcinoma (Gp200) in samples from Human. It has been validated for IHC and FACS.

Quick Overview for Renal Cell Carcinoma (Gp200) (AA 314-410) antibody (ABIN7863513)

Target: Renal Cell Carcinoma (Gp200)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: CA9-3405

Product Details

Binding Specificity: AA 314-410

Purpose: Renal Cell Carcinoma (Carbonic Anhydrase IX) Antibody

Specificity: Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human CA9 protein fragment (around aa 314-410) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details

Target: Renal Cell Carcinoma (Gp200)

Alternative Name: Renal Cell Carcinoma

Background:

CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, Carbonic anhydrase IX, G250, Membrane antigen MN, P54/58N, pMW1, RCC-associated antigen G250, Renal cell carcinoma-associated antigen G250

U87 cells. Normal kidney or renal cell carcinoma (IHC).

Molecular Weight: 55kDa

Gene ID: 768, 63287

UniProt: Q16790