Recombinant CDKN1B antibody

Catalog No. ABIN7857776

This Mouse Monoclonal antibody specifically detects CDKN1B in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant CDKN1B antibody (ABIN7857776)

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDKN1B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: R1B4

Product Details anti-CDKN1B Antibody

Expression System: CHO Cells

Purpose: Recombinant p27Kip1 (Mitotic Inhibitor / Suppressor Protein) Antibody

Specificity: P27 protein, also known as kinase inhibitory protein 1 (Kip1), is a polypeptide which binds to cyclin E/cdk2 complexes (but not to cdk2 alone) and is detected in purified extracts of growth-arrested cells. p27 protein constrains cell proliferation by setting the threshold level of cyclin E necessary to activate cdk2. The 27kD protein is also present in proliferating cells but only in a sequestered form when it is unavailable to interact with cyclin E/cdk2 complexes. It is likely that cyclin D complexed with catalytically inactive cdk4 is sufficient to sequester p27 and titrate its function. The presence of bound p27 in proliferating cells suggests that its role may not be restricted to inducing cell cycle arrest but to also set the cyclin E threshold for execution of the G1 to S phase transition during each mitotic cycle.

Purification: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogen: Prokaryotic recombinant antigen corresponding to the full length p27 molecule

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CDKN1B

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Alternative Name: p27Kip1

Background:

CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, KIP1, MEN1B, MEN4

ZR75, MCF7, MDA-MB-231 or SK-BR-3 cells. Human placenta,Brain, tonsil or spleen.

Molecular Weight: 25-26kDa

Gene ID: 1027, 238990

UniProt: P46527

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy