Recombinant EpCAM antibody (Extracellular Domain)

Catalog No. ABIN7864245

The Rabbit Monoclonal anti-EpCAM antibody is suitable to detect EpCAM in samples from Human and Guinea Pig. It has been validated for WB and IHC.

Quick Overview for Recombinant EpCAM antibody (Extracellular Domain) (ABIN7864245)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Antibody Type: Recombinant Antibody

Reactivity: Human, Guinea Pig

Host: Rabbit

Clonality: Monoclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: EGP40-8191R

Product Details anti-EpCAM Antibody

Binding Specificity: AA 100-224, Extracellular Domain

Purpose: Recombinant EpCAM / CD326 (Epithelial Marker) Antibody

Specificity: Epitope of this MAb is mapped between aa 202-212 of EGP40, which is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Purification: 200ug/ml of Ab purified by Protein A.

Immunogen: Recombinant human EpCAM fragment from extracellular domain (around aa100-224) (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: EpCAM

Background:

Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, KSA, TACD1, TROP1, Tumor-associated Calcium Signal Transducer 1 (TACSTD1)

Human breast tumor. Human Lung, Human Intestine, Guinea pig Lung or Guinea pig Intestine

Molecular Weight: 40-43kDa

Gene ID: 4072, 542050

UniProt: P16422