IL-6 antibody (AA 1-193)

Catalog No. ABIN7858342

The Mouse Monoclonal anti-IL-6 antibody (Clone IL18-4622) (ABIN7858342) specifically detects IL-6 in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for IL-6 antibody (AA 1-193) (ABIN7858342)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: IL18-4622

Product Details anti-IL-6 Antibody

Binding Specificity: AA 1-193

Purpose: IL-6 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) Antibody

Specificity: Four structurally related IL-1 receptor ligands have been described. These include three agonists designated IL-1±, IL-1≤ and IL-1≥/IL-18 and a specific receptor antagonist, IL-1R±. IL-1± and IL-1≤ play critical roles in the regulation of the immune response and inflammation, serving as activators of T and B lymphocytes and NK (natural killer) cells. IL-18 (also referred to as IL-1≥) has been shown to augment the secretion of IFN-≥ from T lymphocytes and increase NK cell activity in spleen cells. IL-18 exhibits 19 % and 12 % identity with IL-1± and IL-1≤ respectively over the 12 ≤-strands of the ≤-trefoil fold domain, which is a signature feature of the IL-1 family. The unusual leader sequence of IL-18 may be analogous to the IL-1≤ pro-domain which must be cleaved by the serine protease ICE for optimal secretion and biological activity. Originally described as IGIF (IFN-≥-inducing factor), IL-18 is induced by mouse liver subsequent to challenge with lipopolysaccharide (LPS).

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa1-193) of human IL-18 protein (exact sequence is proprietary)

Isotype: IgG2, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL-6

Background:

Iboctadekin, IFN gamma inducing factor, IGIF, IL1 gamma, IL18, IL18 protein, IL1F4, IL1g, IL1gamma, Interleukin 18, Interleukin-1 gamma, MGC12320

A549 cells, Human heart or adrenal gland. Stimulated peritoneal macrophages.

Molecular Weight: 18kDa

Gene ID: 3606, 83077

UniProt: Q14116

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome