HNF1B antibody (AA 1-200)

Catalog No. ABIN7858513

The Mouse Monoclonal anti-HNF1B antibody (Clone HNF1B-9519) (ABIN7858513) specifically detects HNF1B in IHC. The antibody is reactive with Human samples.

Quick Overview for HNF1B antibody (AA 1-200) (ABIN7858513)

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HNF1B antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: HNF1B-9519

Product Details anti-HNF1B Antibody

Binding Specificity: AA 1-200

Purpose: Hepatocyte nuclear factor 1 beta (HNF1b) / Transcription Factor 2 (TCF2) Antibody

Specificity: HNF-1 (a and b), HNF-3 (a, b and g), HNF-4 (a and g) and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. HNF-3a, HNF-3b and HNF-3g regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3a and HNF-3b have also been shown to be involved in gastrulation events such as body axis formation. HNF-4a and HNF-4g have been shown to be important for early embryo development. HNF-4a is expressed in liver, kidney, pancreas, small intestine, testis and colon, and HNF-4g is expressed in each of these tissues except liver. HNF-6 has been shown to bind to the promoter of HNF-3b, which indicates a potential role of HNF-6 in gut endoderm epithelial cell differentiation. Evidence suggests that HNF-6 may also be a transcriptional activator for at least 22 other hepatocyte enriched genes, including cytochrome P450 2C13 and a-1 antitrypsin.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa1-200) of human HNF1B protein (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for HNF1B

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Alternative Name: Hepatocyte nuclear factor 1 beta

Background:

T2D, FJHN, HNF2, LFB3, RCAD, TCF2, HPC11, LF-B3, MODY5, TCF-2, VHNF1, ADTKD3, HNF-1B, HNF1beta, HNF-1-beta

Human kidney, pancreas or liver tissue.

Molecular Weight: 61kDa

Gene ID: 6928

UniProt: P35680

Pathways: Hormone Transport, Stem Cell Maintenance, Tube Formation