Recombinant TYMS antibody (AA 1-200)

Catalog No. ABIN7858722

The Rabbit Monoclonal anti-TYMS antibody is suitable to detect TYMS in samples from Human. It has been validated for WB and IHC.

Quick Overview for Recombinant TYMS antibody (AA 1-200) (ABIN7858722)

Target: TYMS (Thymidylate Synthetase (TYMS))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This TYMS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: TYMS-9091R

Product Details anti-TYMS Antibody

Binding Specificity: AA 1-200

Purpose: Recombinant Thymidylate Synthase (5-FU Resistance Marker) Antibody

Specificity: It recognizes a protein of 36 kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human thymidylate synthase protein fragment (around aa 1-200) (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months.,Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for TYMS

Target: TYMS (Thymidylate Synthetase (TYMS))

Alternative Name: Thymidylate Synthase

Background:

dTMP synthase, TMS, TS, TSase, TYMS protein, Tyms thymidylate synthetase

5-FU-resistant colon carcinoma cell lines (NCI H630R10|MCF-Ad5 and MCF-Ad10. Testicular carcinomas.|MOLT4, Ramos|NCI H630R1), 5-FU-resistant breast cancer cell lines, HeLa, Jurkat.

Molecular Weight: 36kDa

Gene ID: 7298, 369762

UniProt: P04818

Pathways: Mitotic G1-G1/S Phases