Recombinant LEF1 antibody (AA 1-399)

Catalog No. ABIN7865002

This Mouse Monoclonal antibody specifically detects LEF1 in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant LEF1 antibody (AA 1-399) (ABIN7865002)

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LEF1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: RLEF1-8854

Product Details anti-LEF1 Antibody

Binding Specificity: AA 1-399

Purpose: Recombinant LEF1 / TCF1 alpha (Transcription Factor) Antibody

Specificity: Lymphoid Enhancing Factor 1 (LEF1) is a transcription factor that belongs to the TCF/LEF family. LEF1 participates as a regulator in Wnt signaling pathways. LEF1 is an important factor in lymphopoiesis and is expressed normally in T and pro-B cells but not expressed in mature B cells. Anti-LEF1 may be used as an aid for differentiation of chronic lymphocytic leukemia/small lymphocytic lymphoma from other small B cell lymphomas.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa1-399) of human LEF1 protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide store at 2 to 8°C. Antibody without azide store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for LEF1

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Alternative Name: LEF1

Background:

Lymphoid enhancer-binding factor 1 (LEF1), T cell specific transcription factor 1 alpha (TCF1a), TCF1 alpha, TCF10, TCF7L3, Transcription factor T cell specific 1 alpha

Human tonsil and thymus.

Molecular Weight: 44kDa

Gene ID: 51176, 726506

UniProt: Q9UJU2

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding